尼妥珠单抗对人食管鳞癌KYSE30细胞株的体外放射增敏作用

摘要

Objective To investigate the radiosensitizing effect of nimotuzumab (h‐R3 ) on human esophageal squamous cancer KYSE30 cell line in vitro .Methods The radiation test was performed in cultured human esophgeal squamous cancer KYSE30 cell line in vitro .The cultured cells were divided into 5 groups .Group C was taken as blank control .Group R was treated with radiation only .Group H1 was treated by h‐R3 at 24 hours before radiation .Group H2 was treated by h‐R3 at 12 hours before radiation .Group H3 was treated by h‐R3 during radiation .The growth‐inhibiting effect was examined by MTS analysis .The clone formation assay was used for detecting and calculating the relevant parameters including the average lethal dose (D0 ) and quasi‐threshold dose (Dq ) .Cell apoptosis and cell cycle distribution after 6 Gy X‐ray radiation were examined with flow cytometry . Results h‐R3 inhibited in vitro the grow th of KYSE30 cell line in the time‐ and dose‐dependent manners .The value of IC20 was 98.88 μg/ml at 24 hours after h‐R3 treatment .Under the effect of h‐R3 for 24 hours ,the radiosensitivity enhancement ratio was 1.43 in group H1 ,1.22 in group H2 and 0 .95 in group H3 .The cell percentage of G2/M stage was increased ,that of S stage was decreased and apoptosis rate was elevated in groups of H1 ,H2 and H3 .Conclusion h‐R3 used before radiotherapy can augment the radiosenditivity of human esophgeal squamous cancer KYSE30 cell line in vitro .%目的：探讨尼妥珠单抗（h‐R3）对体外人食管鳞癌KYSE30细胞株的放射增敏效应。方法采用培养的人食管鳞癌KYSE30细胞株行体外放射实验，分为五组：C组为空白对照；R组仅行照射；H1组于放疗前24 h加用h‐R3；H2组放疗前12 h加用h‐R3；H3组放疗时加用h‐R3。M T S法检测KYSE30细胞株的生长抑制；平板克隆形成实验检测并计算平均致死剂量（D0）和准阈剂量（Dq）等相关参数；流式细胞术检测6 Gy照射下各组的细胞凋亡及细胞周期分布情况。结果 h‐R3对人食管鳞癌KYSE30细胞株增殖呈时间和剂量依赖性的抑制作用。h‐R3作用24 h后细胞增殖20％的药物浓度为98.88μg／ml。在100μg／ml h‐R3作用下，H1组放射增敏比（SER）为1.43，H2组的SER为1.22，H3组的SER为0.95。 H1、H2和 H3组的 G2／M 期比例均增高、S期比例降低、KYSE30细胞凋亡率提高。结论放疗前给予h‐R3能增强食管癌KYSE30细胞株的放射敏感性。