Rho型 GTPase 激活蛋白 MoBem2在稻瘟病菌分生孢子形态建成中的功能

摘要

为了研究Rho型GTPase激活蛋白编码基因MoBEM2(MGG 09303)在稻瘟病菌致病过程中的作用,利用基因敲除方法进行了缺失突变。结果显示该基因缺失突变体产孢量显著下降40%左右,利用荧光增白剂( CFW)染色发现孢子形状变得畸形,形成单个隔膜孢子,约为40%左右,而野生型超过90%均为正常孢子；另外,该基因的缺失导致突变体在培养8h时附着胞形成率显著下降,随着时间的延长,突变体附着胞形成率逐渐上升到野生型程度。可见,MoBem2蛋白调控稻瘟病菌分生孢子的形态建成,延缓分生孢子萌发过程中附着胞的形成,但对稻瘟病菌的致病性没有影响。%To study the function of Rho GTPase activated protein coding gene MoBEM2 (MGG 09303) in conidium morphogenesis, the deletion mutant was identified using the knock-out method in rice blast fungus Magnaporthe oryzae. The deletion mutant produced less conidia ( reduced by 40%) , and staining by calcofluor white ( CFW) showed that fourty per-cent of the conidia were uniseptate in contrast to ninety percent normal three-celled conidia of wild type. The appressorium formation was slowed down significantly at 8 h, but was restored over time. It is indicated MoBem2 is involved in conidium morphogenesis and appressorium formation.