为了研究戊糖片球菌SR2-6对Caco-2细胞的黏附作用，采用不同的化学试剂和酶处理SR2-6，测定其表面成分黏附性质变化，并提取戊糖片球菌表面的细胞黏附素，进行成分和结构分析。同时测量了Caco-2细胞MUC3基因表达量的变化。结果显示，戊糖片球菌 SR2-6能明显上调 Caco-2细胞 MUC3基因表达；戊糖片球菌SR2-6的肽聚糖参与了Caco-2细胞黏附的主要过程，该肽聚糖为α型肽聚糖，提取物主要由谷氨酸、丙氨酸、天冬氨酸、赖氨酸组成，总糖含量为（9.42±1.55）％，蛋白质含量为（4.61±1.88）％。戊糖片球菌SR2-6肽聚糖是一种抑制性黏附素，浓度越高，对SR2-6黏附Caco-2细胞的抑制越强，同时上调MUC3基因表达的作用也越强。%To study the adhesion of Pediococcus pentosaceus SR2-6 to Caco-2 cells, SR2-6 was treated differently with chemical agents and enzymes. The adhesin involved in the adhesion was extracted and the components and structures were analyzed. Peptidoglycan of SR2-6 exerted a major role in the adhesion processes. The peptidoglycan, demonstrated as theα-type, was a kind of inhibitory adhesin, consisting of glutamic acid, malonic acid, aspartic acid and lysine,with total sugar content of (9.42±1. 55)% and protein content of (4.61±1. 88)%. The higher the concentration of peptidoglycan, the stronger the inhibition against the adhesions of SR2-6 to Salmonella and Caco-2 cells, and the stronger the up-regulation of the MUC3 gene. .
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