水稻粒型基因GW2和GS3遗传效应分析

摘要

In this study, GW2 and GS3 genes antisense and overexpression vectors were constructed and transformed with the background materials of big grain variety TD70 and small grain variety Kasalath. The transformed TD70 plants with GW2 ( GW2K ) or GS3 ( GS3K ) gene derived from Kasalath plant exhibited reduced grain weight, and GW2K gene contribu-ted more to the reduction than GS3K gene did. The transformed Kasalath plants with GW2K and GS3K antisense genes showed increased grain weight, and GW2K gene contributed more than GS3K gene did. The transformed Kasalath plants with GW2 ( GW2T ) gene derived from TD70 showed increased 1000-grain weight while the transformed Kasalath plants with GS3 ( GS3T ) gene did not, suggesting that the GW2 and GS3 genes exert different genetic effects in different backgrounds and the contribution of GW2 to grain weight in the same background is greater than that of GS3.%以大粒品种TD70和小粒品种Kasalath为背景材料,分别对其中的GW2和GS3基因进行了反义和超表达载体的构建和转化.结果表明,Kasalath来源的GW2(GW2K)和GS3(GS3K)转化TD70的超表达材料籽粒质量均降低,且GW2K的贡献大于GS3K;GW2K和GS3K转化Kasalath的反义表达材料籽粒质量均增加,GW2K的贡献大于GS3K;TD70来源的GW2(GW2T)转化Kasalath的超表达材料千粒质量显著增加,而TD70来源的GS3(GS3T)转化Kasalath的超表达材料粒型和籽粒质量没有显著变化,说明不同遗传背景中GW2和GS3的基因效应有差异,但同一背景下GW2对籽粒质量的贡献大于GS3.