IL-10及TNF-α在慢性阻塞性肺疾病急性加重期患者血清及痰液中升高的意义

摘要

目的 观察白介素-10(IL-10)及肿瘤坏死因子-α(TNF-α)在慢性阻塞性肺疾病急性加重期(AECOPD)患者血清及痰液的表达,从而探讨AECOPD与免疫机制巾细胞因子的关系.方法 收集26名AECOPD患者作为观察组,22名健康体检者作为对照,用ELISA法分别检测其血清及痰液IL-10及TNF-α的含量.结果 观察组患者血清及痰液的IL-10含量均较对照组升高,但两组患者的血清IL-10含量差异无统计学意义(P=0.071),而观察组的痰液IL-10显著高于对照组(P=0.034):观察组的TNF-α含量均比对照组升高,两组患者血清和痰液TNF-α水平差异具有统计学意义(P=0.023,P=0.008).结论 IL-10与TNF-α升高可能与AECOPD的免疫机制有关.%Objective To observe the quantities of interleukin-10 (IL-10) and tumor necrosis factor alpha (TNF- α)in blood serum and sputum in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD),so as to explore the relation between immunologic mechanism of AECOPD and cytokines expressions.Methods 26 AECOPD patients were divided as observe group and 22 health people as control group.The quantities of IL-10 and TNF-α in blood serum and sputum were tested by ELISA. Results IL-10 in observe group increased and the difference of IL-10 in sputum between two groups was statistically significant(P=0.034 )but not in blood serum( P=0.071 ).TNF- α in observe group also increased obviously and the difference in blood serum and sputum between two groups was both statistically significant(P=0.023,P=0.008). Conclusion IL-10 and TNF-α increased in AECOPD, possibly showing correlation between the two cytokines and the disease.