T细胞α型趋化因子/CXC趋化因子受体-3信号通路在胃癌组织中的表达及其意义

摘要

目的 探讨T细胞α型趋化因子(I-TAC)及CXC趋化因子受体3(CXCR3)在胃癌组织中的表达水平及临床意义.方法 选取2013年1月至2016年1月本院普外科手术切除或黏膜活检所取的胃癌组织40例,相应的癌旁胃组织40例及正常胃黏膜组织20例,采用S-P免疫组织化学技术及逆转录聚合酶链反应技术分别检测I-TAC及CXCR3在三个研究组中的表达水平;并分析I-TAC及CXCR3mRNA的表达与常见临床病理参数的关系.结果 胃癌组织中I-TAC及CXCR3的mRNA和蛋白质表达水平均高于癌旁胃组织及正常胃组织.免疫组化结果提示,I-TAC在胃癌组中阳性表达率(80.0％)高于癌旁组(52.5％)及正常胃组织组(55.0％),差异有统计学意义(P＜0.05);CXCR3在胃癌组中阳性表达率(67.5％)高于癌旁组(45.0％)及正常胃组织(40.0％),差异有统计学意义(P＜0.01).临床病理因素分析提示,CXCR3的表达与胃癌组织的分化程度、淋巴结转移等呈正相关(P＜0.05).结论 胃癌组织中存在I-TAC及其受体CXCR3的特异性激活,提示I-TAC/CXCR3信号传导通路在胃癌发生及侵袭过程中发挥重要作用,可以作为一个诊断和治疗的分子靶点.%Objective To investigate the expression and clinical significance of I-TAC and its receptor CXCR3 in gastric carcinoma tissue.Methods 40 cases of gastric cancer tissue,40 cases of para-carcinoma tissue,and 20 cases of normal gastric tissues from patients undergoing exairesis or biopsy during January 2013 and January 2016 were collected.The expression of I-TAC,CXCR3 in surgical specimens were determined by reverse transcription-polymerase chain reaction and immunohistochemistry respectively.The association of I-TAC,CXCR3,and clinicopathological characteristics were analyzed.Results mRNA expression of I-TAC and CXCR3 in gastric cancer tissue were significantly higher than those in normal gastric tissue and para-carcinoma tissue.The positive expression rate of I-TAC in gastric cancer tissue,para-carcinoma tissue,and normal gastric tissue were 80.0％,52.5％,55.0％ respectively,with statistically significant difference (P＜0.05).The positive expression rate of CXCR3 in gastric cancer tissue was 67.5％,significantly higher than 45.0％ in para-carcinoma tissue and 40.0％ in normal gastric tissue,with statistically significant difference (P＜0.01).The expression of CXCR3 in the tissue of gastric carcinoma was positively related to histologic differentiation,lymph node metastasis.Conclusion I-TAC and CXCR3 are highly activated in gastric cancer tissue,which indicates that I-TAC/CXCR3 signaling pathway may play a important role in the process of development and metastasis,so it may be used as a therapeutic target.