电针对大鼠脊髓损伤后反应性星形胶质细胞增生的影响

摘要

Objective To study effect of electroacupunture on reactive astrogliosis of rats after spinal cord injury. Methods A total of 36 SD rats were randomly divided into the sham group, model group and electroacupuncture group, 12 rats in each group. The sham group only underwent laminectomy without spinal cord injury, and the impactor was used to establish spinal cord injury model in model group and electroacupuncture group. The electroacupuncture group was given electroacupuncture after operation, while the rest groups were given the same condition of grabing. The Basso Beattie Bresnahan (BBB) scores of rats were recorded daily after operation and rats were sacrificed on the 14th day after administration. Nissl staining was used to observe the morphology of neurons. The expression of GFAP was detected by immunofluorescence. The expression of GFAP protein was detected by Western blot. Results The fifth days after the intervention, the BBB score of the electroacupuncture group was significantly higher than that of the model group (P<0.05). Compared with the model group, the expression of GFAP in electroacupuncture group significantly decreased (F=23.831, P<0.001), the expression of GFAP protein in electroacupuncture group significantly decreased (F=15.883, P<0.001), the expression of IL-1β and TNF-α in electroacupuncture group significantly decreased (F=82.773, 113.487, P<0.001). Conclusions The electroacupuncture can inhibit the expression GFAP and astrogliosis to inhibit inflamation after spinal cord injury in rats, which is conducive to the repair of neurons and the recovery of limb motor function in rats.%目的 观察电针对脊髓损伤后大鼠反应性星形胶质细胞增生的影响.方法 将36只SD大鼠按随机数字表法分为假手术组、模型组、电针组,每组12只.假手术组仅行椎板切除术而不损伤脊髓,模型组和电针组采用脊髓打击器制备脊髓损伤模型.造模后电针组给予电针干预,其余各组给予同等条件抓取.造模后每日对大鼠进行脊髓损伤行为学评分(Basso Beattie Bresnahan,BBB)评分.造模后14 d取材.采用尼氏染色法观察神经元形态,采用免疫荧光染色检测大鼠脊髓组织胶质纤维酸性蛋白(glial fibrillary acid protein,GFAP)表达情况,采用Western blot检测大鼠脊髓组织GFAP蛋白表达水平,采用ELISA检测血清炎性细胞因子IL-1β、TNF-α水平.结果 造模后第5天起,电针组BBB肢体运动功能评分高于模型组(P<0.05);与模型组比较,电针组大鼠脊髓组织GFAP阳性反应平均光密度降低(F=23.831,P<0.001),GFAP蛋白表达降低(F=15.883,P<0.001),血清IL-1β、TNF-α水平降低(F=82.773、113.487,P<0.001).结论 电针可抑制大鼠脊髓损伤后GFAP表达和反应性星形胶质细胞增生,抑制炎症,从而有利于神经系统修复和大鼠肢体运动功能恢复.