人体肠道菌群研究中粪便样品制备与保存研究进展

摘要

随着高通量测序技术(next generation sequencing,NGS)的飞速发展,肠道菌群得以被更深入的研究.肠道内容物和粪便样品具有取样便捷、代表性强等特点,因此常被作为肠道菌群研究中的主要研究对象;而样品的收集和保存方法很大程度上影响其内部菌群结构和多样性,从而决定了后续测序分析的准确性.该文将就现今常用的肠道菌群研究中粪便样品的制备和保存方法进行综述.%With the rapid development of high-throughput sequencing ( NGS) technique, intestinal microbiome could be studied more deeply. Intestinal contents and fecal samples have the characteristics of con-venient sampling and strong representation, so they are often used as the main research objects in the study of intestinal microbiota. The method of collecting and storage of samples are very important to affect the internal flora structure and diversity, which determines the accuracy of subsequent sequencing analysis. This review summarizes the sampling and storage methods of fecal samples in the study of intestinal microbiome.