基因芯片技术在乙型肝炎病毒分型和耐药突变检测中的应用

摘要

目的 通过基因芯片检测系统,快速检测临床样品中乙型肝炎病毒的基因亚型和耐药突变情况.方法 用基因芯片法检测352例临床样品,对慢性乙型肝炎患者感染的HBV亚型及耐药情况进行分析.结果 在352例临床病例中,共检出耐药突变病例124例,检出率为35.7%.发现3种基因亚型,其中B型222例,占63.8%;C型115例,占33.0%;D型1例,占0.3%;B、C混合感染9例,占2.6%;型别未分1例,占0.3%.结论 PCR与膜芯片杂交技术可检测乙型肝炎病毒基因亚型及耐药突变类型,并具有快速、高通量、敏感的特点,适合各临床医院开展应用.%Objective To rapidly detect the genopypes of HBV and drug-resistance mutation inclinical samples by means of gene chip detection system.Methods 352 cases of clinical samples were collected.The genopypes of HBV and drug-resistance mutation in the clinical samples were analyzedwith gene chip detection system.Results The detection rate of drug-resistance mutation cases was 35.7%(124/325).Three subtypes were found,in which genotype B was 63.8%(222/352),genotype C was 33.0%(115/352),and genotype D was 0.3%'(1/352).Mixed infection of genotype B and C accounted for 2.6%,and unidentified case was 1(0.3%).Conclusion Combined application of PCR and gene chip technique can be used to detect HBV genotyping and drug-resistance mutation,and it is specific,sensitive and convenient,suitable for application in different levels of hospitals.