多药耐药铜绿假单胞菌菌株亲缘性分析

摘要

目的 了解多药耐药铜绿假单胞菌(multi-drug-resistant pseudomonas aeruginosa.MDRPA)分离株亲缘性.方法 采用PCR检测20株MDRPA菌34种耐药基因,并作聚类分析.结果 20株MDRPA菌中,TEM、OXA-2群、OXA-10群、CARB、rmtB、aac(3)-Ⅱ、aac(6')-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA、merA、qacE△1-sull基因阳性率分别为30%、5%、5%、25%、35%,10%、25%、30%、35%、15%、30%、35%、50%,oprD2缺失率为100%.聚类分析显示存在克隆传播现象.结论 TEM、CARB、rmtB、aac(3)-Ⅱ、aac(6])-Ⅰ b、aac(6')-Ⅱ、ant(3")-Ⅰ、ant(2")-Ⅰ、cmlA,merA、qacE△1-sull基因检出率高,MDRPA菌可导致克隆传播医院内感染.%Objective To investigate the affinity of muhidrug resistant isolates of Pseudomonas aeruginosa. Methods The 34 kinds of drug resistance genes of 20 strains of multidrug resistant Pseud-omonas aeruginosa (MDRPA) were detected by applying polymerase chain reaction (PCR) and cluster analysis. Results The positive rates of TEM, OXA-2group, OXA-10group, CARB, rmtB, aac(3)- Ⅱ , aac(6')- Ⅰ b,aac(6')- Ⅱ ,ant(3"-Ⅰ ,ant(2")- Ⅰ ,cmlA,merA,qacE△l-sull were 30% ,5% ,5% ,25%, 35%, 10% ,25% ,30% ,35%, 15%,30% ,35% and 50%, respectively. The deletion rate of oprD2 gene was 100%. Cluster analysis showed that the clone transmission occurred. Conclusion There are very high detection rates of TEM,CARB,rmtB,aac(3)- Ⅱ ,aac(6')- Ⅰ b,aac(6')- Ⅱ ,ant(3")- Ⅰ ,ant(2")- Ⅰ, cmlA,merA,qacE△l-sull genes in MDRPA. The MDRPA can induce clone transmitted hospital infec-tion.