To investigate the relationship of gynecological diseases and human papillomavirus (HPV)infection in Shi jingshan area in Beijing, and establish the epidemiological data base of HPV infection in female with different in gynecological dis ea ses and at different ages. Methods 1 012 cases of specimens from patients and 500 cases from healthy females were detected with reverse dot blot,and the data were analyzed by statistical software SPSS13.0. Results Among the specimens from patients, 351 (34.7 ％) cases were positive. Among the positive specimens, 51 (14. 5) cases were with double infection, 22 (6.3 ％) were with multiple infection,226(22.3％) were with high risk infection and 140 (39.9％) were with HPV16 infection only. Among the spec imens from healthy females, 93(18.6 ％) cases were positive and the rate of high risk infection was 21.6 ％. Tthere was significant difference of HPV infection among females at different ages, with different sexual history and gynecologic diseases. Conclusion HPV genotyping could provide important reference for the etiology analysis and prediction of uterine cervix cancer and female geni tal tract cancer,and offer evidence for the prophylaxis, diagnosis,and therapy of uterine cervix cancer at early stagea.%目的 探讨北京石景山地区妇科疾病与人乳头瘤病毒感染(HPV)的关系,建立本地区不同妇科疾病和不同年龄段妇女HPV感染的流行病学资料库.方法 应用反向点杂交技术分别对疾病组1 012例患者标本和正常组500例标本进行HPV检测,所得数据用SPSS13.0统计软件进行分析.结果 疾病组1 012例标本中,阳性标本351例,感染率为34.7%;其中双重感染51例,占阳性标本的14.5%;多重感染22例,占阳性标本的6.3%;而高危感染226例,感染率为22.3%,占阳性标本的64.4%;单纯HPV16亚型感染140例,占所有阳性标本的39.9%.正常组500例标本中,阳性标本93例,感染率为18.6%;高危型所占比例为21.6%.不同年龄段、不同性生活史和不同妇科疾病的HPV感染检出率都有显著性差异.结论 HPV DNA 的基因分型可为宫颈癌及女性生殖道肿瘤的病因学和癌前预报提供重要的参考价值,为高风险人群的早预防、早诊断、早治疗提供依据.
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