实时荧光RT-PCR检测不同标本中甲型H1N1流感病毒核酸的临床意义

摘要

Objective To explore the relationship of influenza A virus ( H1N1) nucleic acid with rapid diagnosis of influenza A ( H1N1) and the progress of disease course by the detection of viral nucleic acid in different specimens from patients with influenza A ( H1N1). Methods Fluorescent real-time reverse transcription polymerase chain reaction (RT-PCR) was performed to detect influenza A virus ( H1N1) nucleic acid in throat swabs, plasma and secretion of lower respiratory tract from 118 patients with influenza A ( H1N1) 48 hours after onset and (or) during convalescence respectively. Results Viral nucleic acid was detected in throat swabs from all 118 patients 48 hours after onset,secretion of lower respiratory tract from 23 severe cases and plasma from 15 severe cases. Coriclusion Fluorescent real -time RT-PCR could be used for the rapid detection of influenza A virus ( H1N1) infection. Influenza A virus (H1N1) nucleic acid,when be detected in plasma,may be the indication of the severe cases.%目的 通过检测甲型H1N1流感感染患者不同标本中甲型H1N1流感病毒核酸,了解其对甲型H1N1流感的快速诊断及病程进展的关系.方法 118例甲型H1N1流感患者,采用实时荧光逆转录聚合酶链反应法(RT-PCR)分别检测发病48 h后和(或)恢复期咽拭子、血浆及下呼吸道分泌物中甲型H1N1流感病毒核酸.结果 118例甲型H1N1流感患者发病48 h后咽拭子甲型H1N1流感病毒核酸阳性;23例(23/40)重症患者下呼吸道分泌物中甲型H1N1流感病毒核酸阳性;15例(15/40)重症患者血浆中检测到甲型H1N1流感病毒核酸.结论 实时荧光RT-PCR方法可以快速检测甲型H1N1流感病毒感染,血浆中甲型H1N1流感病毒核酸阳性可能是重症患者的标志.