非侵入性诊断筛查甲状腺恶性肿瘤的可行性探讨

摘要

Objective To establish serum marker pattern for thyroid malignancy and discuss the feasibility of non-traumatic diagnosis for thyroid malignancy to investigate sensitive markers for the screening of thyroid malignancy .Methods Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to analyze serum protein profile of 30 cases highly suspected thyroid malignancy and 43 cases of healthy control .The data were analyzed by Biomarkers Pattern Soft (BPS) to establish diagnosis model .Results With the 5 markers,the training set could differentiate the highly suspected thyroid malignancy from the healthy control with the sensibility of 86.7%,specialty of 91.2% and total accuracy of 93.75% .Conclusion SELDI-TOF-MS and bioinformatics technology might be helpful to establish a biomarker pattern for more consummate medical examination.%目的 应用可用于健康筛查的甲状腺恶性肿瘤标志物模型,探讨非侵入性诊断甲状腺恶性肿瘤的可行性,以期为健康体检筛查提供特异性较强的肿瘤标志物.方法 采用表面增强激光解析电离-飞行时间质谱(SELDI-TOF-MS)对30例高度怀疑甲状腺恶性肿瘤患者及43例健康者进行血清蛋白质谱分析,应用生物标志物模型软件(BPS)建立判别模型,筛查具有一定特异性的标志物模型,以留一法进行交叉验证.结果 标志物模型交叉验证灵敏度为86.7%,特异度为91.2%,总符合率为93.75%.结论 采用SELDI-TOF-MS技术和生物信息技术建立标志物模型,是非侵入性诊断甲状腺恶性肿瘤的有益尝试,有助于完善健康体检筛查模式.