铜绿假单胞菌群体感应lasI/rhlI基因对其生物被膜形成的影响

摘要

目的 分析铜绿假单胞菌群体感应系统lasI/rhlI基因对生物被膜形成的影响,探讨群体感应系统对生物被膜形成的调控机制.方法 采用结晶紫染色法分析铜绿假单胞菌标准株PAO1及其群体感应系统lasI/rhlI基因缺陷株PA210(△rhlI)、PA214(△lasI)及PA216(△lasI/rhlI)生物被膜的形成能力.结果 铜绿假单胞菌标准株PAO1能形成成熟的生物被膜,基因缺陷株PA214(△lasI)可形成较薄的生物被膜,而PA210(△rhlI)及PA216(△lasI/rhlI)则不能形成生物被膜.结论 lasI/rhlI基因缺陷可影响铜绿假单胞菌生物被膜形成,但rhlI基因对生物被膜形成的影响更为显著.%Objective To analyze the influence of Pseudomonas aeruginosa quorum-sensing system genes lasI/rhlI on bacterial biofilm formation,in order to explore the control mechanism of quorum-sensing system on the biofilm formation.Methods The formation ability of Pseudomonas aeruginosa standard strains PAO1 and quorum-sensing system lasI/rhlI genetic defect strains PA210(ΔrhlI),PA214(/lasI) and PA216(/lasI/rhlI) biofilm was analyzed by using crystal violet dyeing method.Results Pseudomonas aeruginosa standard strains PAO1 formed the mature biofilm,genetic defect strains PA214(/lasl) formed the thin biofilm,and PA210(/rhlI) and PA216(/lasI/rhlI) could not form biofilm.Conclusion lasI/rhlI genetic defects can affect the formation of Pseudomonas aeruginosa biofilm,but rhll gene influence more significant on the formation of biofilm.