同一实验室多台血液分析仪的比对结果分析

摘要

目的：评价多台血液分析仪检测结果的一致性。方法采用NCCLS文件EP9-A2指南、Passing-Bablok回归分析法和t检验，使用115例患者标本对5台血液分析仪（XE-2100，XT-1800i ，XS-1000i ，BC-5300，ABX Pentra80）的血红蛋白（HGB）、红细胞压积（HCT）、红细胞计数（RBC）、白细胞计数（WBC）、血小板计数（PLT）进行了比较，其中 Sysmex XE-2100血液分析仪作为参比仪器。结果在所有比对项目 HGB、HCT、RBC 、WBC、PLT 中，这几台血液分析仪显示出了非常好的相关性（r＞0．97），但有3台血液分析仪的个别项目产生了比例系统误差和/或恒定系统误差，包括XT-1800i血液分析仪的 HCT项目，XS-1000i血液分析仪的 HCT、RBC项目，ABX80血液分析仪的RBC项目。结论通过 Passing-Bablok回归分析法联合 t检验能识别出两台仪器比对结果的系统误差，不可以使用简单的相关系数评价血液分析仪比对结果的一致性。%Objective To evaluate the consistency of the test results detected by multiple automated hematology analyzers .Meth-ods The EP9-A2 guideline of NCCLS ,Passing-Bablok regression analysis and t test were adopted to compare the detection results of hemoglobin(HGB) ,hematocrit (HCT) ,red blood cell count(RBC) ,white blood cell count (WBC) and platelet(PLT) in 115 samples from patients by 5 automated haematology analyzers of the Sysmex XE-2100 ,the Sysmex XT-1800i ,the Sysmex XS-1000i , the Mindray BC-5300 and ABX pentra80 .The Sysmex XE-2100 was used as the reference instrument .Results In the detection of all the compared items including HGB ,HCT ,RBC ,WBC and PLT ,these automated hematology analyzers exhibited very good cor-relation(r>0 .97) ,whereas the individual parameters in 3 automated hematology analyzers appeared the proportional systematic differences and /or constant systematic differences ,including HCT in the sysmex XT-1800i ,HCT and RBC in the sysmex XS-1000i and RBC in the ABX pentra 80 .Conclusion The Passing-Bablok regression analysis combined with t test may identify the system-atic differences of the comparative results of 2 automated haematology analyzers ,the consistency of the comparative results of the automated hematology analyzers can not be evaluated by the simple correlation coefficient .