磺胺类药物对结核分枝杆菌体外抗菌活性的研究

摘要

目的：评价磺胺甲噁唑（SMZ）和甲氧苄啶（TMP）对结核分枝杆菌（MTB）的体外抑菌作用，为临床应用提供依据。方法应用微孔板观察法，测定TMP／SMZ对MTB敏感标准株H37Rv、临床敏感菌株（20株）和临床耐多药（MDR）菌株（30株）的最小抑菌浓度（MIC）。结果标准株H37Rv的MIC值为0．5μg／mL TMP＋9．5μg／mL SMZ。40株（80％）临床分离MTB可以被MIC值为1μg／mL TMP＋19μg／mL SMZ的复合制剂抑制生长，其中包含17株敏感菌株、23株MDR菌株。结论 TMP联合SMZ对M TB菌株有较好的体外抑菌活性。%Objective To evaluate the bacteriostasis of sulfamethoxazole(SMZ)combined with trimethoprim(TMP)against My‐cobacterium tuberculosis(MTB) in vitro ,so as to provide basis for clinical application .Methods The minimal inhibitory concentra‐tion(MIC) value of TMP/SMZ against MTB ,including standard sensitive strain(H37Rv) ,clinical sensitive strains(20 strains) and clinical multiple‐drug‐resistance strains(MDR ,30 strains) ,was detected by using MPP observation method .Results The MIC val‐ue of standard strain H37Rv was TNP(0 .5 μg/mL)+SMZ(9 .5 μg/mL) .The growth of 40 strains(accounted for 80% ) of clinical isolates ,including 17 sensitive strains and 23 MDR strains ,could be inhibited by TMP(1 μg/mL)+ SMZ(19 μg/mL) compound . Conclusion TMP combined with SMZ may has good antibacterial activity for strains of MTB in vitro .