应用改良试剂瓶对提高生化试剂开瓶稳定性的影响

摘要

Objective To study ,analyse and applicate improved reagent bottles′efficacy of improving the stability after opening of biochemical reagents .Methods We divided into observation group and matched groups ,the observation groups were biochemical reagents ,compared with common reagents ,and we tested the stability after opening on Na + ,Cre ,Lp (a) ,ALP ,TBA ,etc .using im‐proved reagent bottles by automatic biochemical analyzer ,and observed their experimental results .Results After the examination , the mixed serum′normal figures and pathological figures of Na + 1 were (134 .6 ± 4 .23) mL and (154 .8 ± 5 .54) mL ,Na+ 2 were (129 .1 ± 1 .5)mL and (149 .1 ± 1 .35) mL ,TBA l were (4 .66 ± 0 .43)mL and (36 .67 ± 3 .17) mL ,TBA 2 were (5 .53 ± 0 .21)mL and (41 .67 ± 1 .13) mL ,Cre 1 were (108 .5 ± 5 .9)mL and (336 .8 ± 19 .0) mL ,Cre 2 were (115 .1 ± 1 .9) and (349 .7 ± 4 .12) mL , ALP l were (148 .3 ± 6 .7) and (324 .7 ± 11 .9) mL ,ALP 2 were (153 .6 ± 1 .6) and (331 .2 ± 4 .6)mL ,Lp (a) 1 were (183 .0 ± 5 .4) and (401 .9 ± 21 .4) mL ,Lp (a) 2 were (201 .1 ± 6 .5) and (429 .1 ± 10 .2)mL .Conclusion The improved reagent bottles had obvi‐ous effect on improving the stability and period of validity after opening the bottles of Na + ,Cre ,Lp (a) ,ALP ,TBA ,etc .,if Reagent formula ingredients do not change ,it will not have a negative impact ,the improved reagent bottles can replace traditional reagent bottles and widely used in Automatic Biochemical Analyzer ,will have broad prospects .%目的：探讨并分析应用改良试剂瓶对提高生化试剂开瓶稳定性的影响。方法将试剂分为观察组与对照组，其中观察组为生化试剂，对照组为普通试剂，利用全自动生化分析仪使用改良试剂瓶对生化试剂钠（Na＋），肌酐（Cre），脂蛋白a[Lp （a）]，碱性磷酸酶（ALP），总胆汁酸（TBA）等进行开瓶稳定性等试验，观察其实验结果。结果经检测，Na＋1的正常值混合血清及病理值混合血清分别为（134．6±4．23）mL和（154．8±5．54）mL ，Na＋2为（129．1±1．5）mL和（149．1±1．35）mL ，TBA l为（4．66±0．43）mL和（36．67±3．17）mL ，TBA 2为（5．53±0．21）mL和（41．67±1．13）mL ，Cre l为（108．5±5．9）mL和（336．8±19．0）mL ，Cre 2为（115．1±1．9）mL和（349．7±4．12）mL ，ALP l为（148．3±6．7）mL和（324．7±11．9）mL ，ALP 2为（153．6±1．6）m L和（331．2±4．6）m L ，L p （a ）1为（183．0±5．4）m L和（401．9±21．4）m L ，L p （a ）2为（201．1±6．5）m L以及（429．1±10．2） mL。结论改良试剂瓶对于提高Na＋，Cre ，Lp（a），ALP ，TBA等生化试剂开盖后的稳定性、有效期等效果显著，试剂配方成分无改变，不会产生负面影响，可替代传统试剂瓶并广泛应用于生化自动化分析，前景广阔。