Objective To perform a comparative research on nucleoside drug resistance mutation and genotypes of chronic hepa‐tits B virus in Shanghai and Lanzhou .Methods The serum samples(Shanghai ,194 cases) and (Lanzhou ,325 cases) were collected . The common 7 drug resistant mutation loci ,genotype B and C were detected by using the PCR‐LDR method .The obtained data were analyzed by using the SPSS17 .0 statistical software .Results (1) Genotype C was the main genotype in two areas (Shanghai 79 .3% ,Lanzhou 81 .4% ) .(2) The mutation rate in Shanghai was higher than that in Lanzhou with statistical difference (48 .9%vs .35 .3% ,P<0 .01) .Lamivudine resistance mutation was predominant in all the mutations of two areas ,accounting for 75 .7%(153/202 ;Shanghai 68/92 ,Lanzhou 85/110);the difference in lamivudine resisrtance mutation loci had statistical difference be‐tween the two areas ;while the adefovir resistance loci was dominated by rtA181T/V ,the area difference had no statistical signifi‐cance(P>0 .05) .(3) The drug resistance mutation had no correlation with genotype .The C genotype mutation rate in Shanghai was higher than that in Lanzhou ,the difference was statistically significant (49 .0% vs .35 .4% ,P<0 .05 ) .(4) 4 kinds of drug re‐sistance rate had no correlation with genotype .Conclusion The genotype distributions are similar in two areas ;Shanghai has a higher drug resistance rate than Lanzhou ;the drug resistance occurrence has no relation with drug resistance type and genotype .%目的：探讨上海和兰州2个地区的慢性乙型肝炎病毒核苷类药物耐药突变和基因型的比较及关系。方法取上海和兰州地区慢性乙型肝炎患者194例和325例的血清，利用聚合酶链反应（PCR）‐连接酶反应检测技术（LDR）法对常见的7种耐药突变位点和B型、C型两种型别进行检测。使用SPSS17．0统计软件对数据进行分析。结果（1）2个地区均以C型为主，上海、兰州分别为79．3％、81．4％。（2）上海地区突变比率（48．9％）高于兰州地区（35．3％），差异有统计学意义（ P＜0．05）。2个地区耐药突变均以拉米夫定突变为主，占75．7％（153／202，上海68／92，兰州85／110），2个地区导致拉米夫定耐药突变位点上差异有统计学意义（P＜0．05）；而2个地区的阿德福韦耐药位点均以 rtA181T／V为主，地区间差异无统计学意义（P＞0．05）。（3）是否耐药突变与基因型无相关性，C基因中上海地区的突变比率（49．0％）高于兰州地区（35．4％），差异有统计学意义（ P＜0．05）。（4）4种耐药比率在与基因型无相关性。结论上海、兰州2个地区在基因型分布上相似，上海地区发生耐药比率更高，2个地区均显示发生耐药与否以及耐药类型与基因无关。
展开▼
