Objective To investigate the effect of repeated use on the stability of HIV quality control serum during cold storage , and put forward measures to deal with .Methods Ten HIV serum from -20 degrees celsius freezer room temperature balance out , 30 minutes to make it completely melted ,randomly arranged in the patient sample ,added sample testing according to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator .The next day ,the above quality control products from 2 to 8 degrees celsius refrigerator ,after 30 minutes at room temperature ,randomly arranged in the patient sample ,add sample testing ac-cording to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator ,repeat the above activities to seventh days .Using repeated measure ,at the same time ,took the average 10 quality control of product values on the same day as a "check-point"compared with daily quality control chart ,observe changes .Results It suggested that in different measurement on the num-ber of days the difference was statistically significant(F=48 .515 ,P<0 .01) ,combined with the quality control chart found ,which was starting from the fourth day quality value "warning"status ,began to appear and the drift of fell out of control .Conclusion HIV quality-control serum at 2 to 8 degrees celsius refrigerator ,used repeatedly during 3 days late stability is affected ,the change of quality control diagram appear drift ,even is out of control .%目的 基于冷冻质控血清复溶后在冷藏期间多次反复使用 ,对人类免疫缺陷病毒(HIV )抗体质控血清稳定性的影响进行探讨及寻找应对措施.方法 将10支HIV质控血清从-20 ℃冰柜取出 ,室温平衡30 min使其完全融化 ,随机排列到病例样本之中 ,按常规样本进行加样检测 ,加样完毕后及时保存至2~8 ℃冰箱 ;次日起 ,上述质控品从2~8 ℃冰箱取出室温平衡30 min后 ,随机排列到病患样本之中 ,按常规样本进行加样检测 ,加样完毕后及时保存至2~8 ℃冰箱 ,重复以上活动至第7天;采用单因素重复测量资料的方差分析进行统计分析 ,同时 ,把同一天的10支质控品检测值取平均值作为一个"检测点"与日常质控图进行比较 ,观察变化情况.结果 单因素重复测量资料的方差分析 ,说明在不同测量天数上差异有统计学意义( F=48 .515 ,P<0 .01 ) ,结合质控图发现 ,从第4天开始质控值开始出现"告警"状态 ,且呈漂移式的下降至失控状态.结论 H IV质控血清冻溶后置于2~8 ℃冰箱冷藏 ,期间反复多次使用 ,3d后稳定性受到影响 ,质控图出现漂移、甚至失控的变化.
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