Adhesive properties of hepatoma cells to collagen Ⅳ coated surfaces

摘要

Objectives: To quantitatively study the adhesive pro-perties of hepatoma cells to collagen Ⅳ coated artifi-cial basement membrane and to investigate the rele-vance of cell adhesive forces to the concentration ofcollagen Ⅳ.Methods: Synchronous G1 and S phase cells were a-chieved using thymine-2-desoxyriboside and cochicinesequential blockage method and double thymine-2-desoxyriboside blockage method respectively. Theadhesive forces of hepatoma cells were investigatedby micropipette aspiration technique.Results: The adhesive forces of hepatoma cells to ar-tificial basement membrane were （107.78±65.44）×10-10N, （182.60±107.88）×10-10N, （298.91±144.13）×10-10N when the concentration of themembrane coated by 1, 2, 5μg/ml collagen Ⅳ re-spectively （P0.001）. The adhesive forces of G1and S phases hepatoma cells to artificial basementmembrane were （275.86±232.80）×10-10N and（161.16±120.40）×10-10N respectively when theconcentration of the membrane coated by 5μg/mlcollagen Ⅳ （P0.001）.Conclusions: The adhesive forces of hepatoma cells toartifical basement membrane in direct proportion tothe concentration of collagen Ⅳ suggests that the in-crease of basement membrane might be conducive tothe chemotactic motion and adhesiveness of tumorcells. G1 phase cells are more capable of adhering tobasement membrane than S phase cells. Hepatomacells, especially G1 phase cells, may survive in bloodcirculation, and sequest and adhere in microcircula-tion, and get through basement membrane for re-mote metastasis.