养心颗粒对易损斑块家兔模型胆固醇7-α-羟化酶和MMP-9含量的影响

摘要

目的:探讨养心颗粒对易损斑块家兔模型胆固醇7-α-羟化酶和MMP-9含量的影响.方法:采用球囊损伤家兔颈总动脉,同时给予家兔高脂饮食喂养8周,8周后注入p53腺病毒载体,将造模成功家兔随机分为模型对照组、养心颗粒组、辛伐他汀组,每组6只,另取6只家兔作为空白对照组,药物治疗2周后进行药物触发,用ELISA法检测胆固醇7-α-羟化酶和MMP-9含量.结果:养心颗粒组与治疗前比较,CYP7A1含量升高、MMP-9含量减少,差异具有统计学意义(P＜0.05).辛伐他汀组与治疗前比较,CYP7A1含量明显升高,差异具有统计学意义(P＜0.05)；两治疗组相互比较,差异均无统计学意义(P＞0.05).结论:养心颗粒可升高易损斑块家兔模型血清CYP7A1含量,降低MMP-9表达水平,这可能是养心颗粒稳定易损斑块的作用机制之一.%To study the effect of Yangxin granules on CYP7A1 and MMP -9 in vulnerable plaque rabbit models. Methods; At first rabbits'carotid artery were induced with an balloon, then these rabbits were fed on high fat diet for 8 weeks. After 8 weeks, p53 gene transfection was injected to plaques,and rabbits were randomly divided into four groups; model contrast group, Yangxin granule group, simvastatin contrast group and blank contrast group. Administration for two weeks. At the end of the 8th weekend and 10th weekend,the contents of CYP7A1 and MMP -9 were examined. Results;On the 10th weekend the content of CYP7A1 increased in treatment groups and simvastatin group than the content before ( P< 0.05) , and the content of MMP—9 decreased in treatment groups than the content before. There was a significant difference(P <0.05) ,but no significant difference between Yangxin granule group and simvastatin group ( P> 0.05). Conclusion; Yangxin granule can increase the content of CYP7A1 and decrease the content of MMP -9 in vulnerable plaque rabbit models,which might be one of the mechanisms for stabilizing vulnerable plaque.