Objective: To observe the effect of Beimu E xiao pill on TSH and ethology in PTU - induced goiter rats with hypothyroidism, and to further explore its action mechanism. Methods: 10 SD rats were randomly selected from 40 rats as the blank control, the rest rats were given PTU(0.15 mg/g) once per day for 40 days to set up the model. The model rats were randomly divided into the model group, the Chinese medication(Beimu E'xiao pill) group, and the western medication(Sodium Levothyroxine) group, with 10 rats in each group. Ethology test (open - field test) and novelty cognitive test were carried out in the rats before the treatment, 10 d, 20 d, and 30 d after the treatment. Morphological changes of thyroid cells were observed and TSH value was measured 30 d after the treatment. Results: PTU could induce goiter concurrence with hypothyroidism in rats; TSH value was significantly decreased in the Chinese medication group compared to that in the western medication group; it can significantly improve the abnormal spatial cognition abiliy in hypothyroidism rats. Conclusion: PTU can lead to goiter concurrence with hypothyroidism in rats, Beimu Exiao pill can promote the decrease of TSH, it can significantly improve the abnormal spatial cognition ability in hypothyroidism rats.%目的:通过丙硫氧嘧啶(PTU)造成大鼠甲状腺肿伴甲减的模型,观察贝牡莪消丸对甲状腺肿伴甲减大鼠TSH值及行为学方面的影响,进而探究该药物对本病的作用机制.方法:选取40只SD大鼠,随机选取其中10只作为空白组,其余30只给予丙硫氧嘧啶0.15 mg/g,每日1次,持续灌胃40天.造模成功后,将30只大鼠随机分为3组,即模型对照组、贝牡莪消丸组、优甲乐组,分别喂以生理盐水、贝牡莪消丸、优甲乐.给药30天后,以10天为1个周期,分别于给药前、给药10天后、给药20天后、给药30天后,进行大鼠行为学指标检测(敞箱实验)及新奇事物认知试验检测,并于给药30天后处死大鼠,镜下观察大鼠甲状腺细胞形态,并取血清测定TSH.结果:丙基硫氧嘧啶能够导致大鼠的甲状腺肿大并发甲减;贝牡莪消丸组的血清TSH值较优甲乐组显著降低;显著改善甲减大鼠异常空间认知能力.结论:PTU能够导致大鼠甲状腺肿大并发甲减,贝牡莪消丸可以促使甲状腺肿伴甲减大鼠TSH水平降低,并且显著改善甲减大鼠异常空间认知能力.
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