葡萄糖诱导型AOX1启动子毕赤酵母突变株B5的筛选

摘要

在毕赤酵母表达系统中,AOX1启动子被广泛应用于驱动异源蛋白的表达,但是AOX1启动子的活性严格依赖于甲醇的诱导.本文成功筛选到一株毕赤酵母紫外诱变随机突变株B5,该突变株可以在葡萄糖碳源中表达Aox酶.利用绿色荧光蛋白GFP作为报告蛋白,B5在葡萄糖中具有AOX1启动子活性,但是在甘油中仍然没有AOX1启动子活性.该突变株提供了一种葡萄糖诱导型的新型AOX1启动子.%In Pichia pastoris, the promoter of the alcohol oxidase 1 (AOX1 ) gene was commonly used to control the expression of heterologous proteins and the induction of A0X1 promoter was strictly dependent on methanol. After mutation, the UV-induced Pichia pastoris mutants B5 was obtained, which was able to express alcohol oxidase when grew on glucose as sole carbon source. However, no alcohol oxidase activity was detectable when grew on glycerol in mutant B5.