高盐方法提取蒙古黄芪基因组DNA比较分析

摘要

为了提取高质量的蒙古黄芪(Astragalus membranaceus)基因组DNA,采用高盐十二烷基硫酸钠(SDS)法、高盐溴化十六烷基三甲基溴化铵(CTAB)法和高盐低pH值法,结果表明高盐CTAB法获得的黄芪基因组DNA纯度高,能用于PCR扩增和限制性内切酶酶切.是提取黄芪基因组总DNA的最佳方法.%High-salt SDS, high-salt CTAB, and high-salt low pH value methods were used to extract high quality Astragalus membranaceus genomic DNA.The results showed that the genomic DNA with the highest quality was extracted by high-salt CTAB method, and was preferable enough to be used as PCR template and digested by restriction enzyme completely; thus high-salt CTAB was the best DNA extraction method.