纤维素降解菌系的筛选及降解稻草条件研究

摘要

以已分离的11株纤维素降解菌为材料,采用滤纸崩解法和透明圈法,初步筛选出4株纤维素降解能力较强的菌株.将这4株单菌进行两两组合.研究混合菌系在9d内的CMC酶活和FPA酶活与单菌发酵之间的异同.结果表明,混合菌发酵CMC酶活和FPA酶活均优于单一菌株;同时筛选出一组具有高降解能力的混合菌体系;并对该混合菌系降解稻草的最适反应温度、最适初始pH值、产生还原糖的时间进行了研究.结果表明,在30℃、pH值4.5、发酵96h时混合菌降解稻草的效果最好.%Four strains with strong degradation ability to cellulose materials were screened out of 11 bacteria using filter paper degradation method and clear halo method.Then a mixed germ with higher degradation ability was obtained as the CMC and FPA enzyme activity of mixed bacteria and pure bacterium was compared.The optimum degrading condition of the mixed germ to rice straw was 30℃, pH 4.5, and fermentation for 96 h.