Fractionation of polysaccharides extracted fiom Sargassum pallidum by membrane technology has been studied.The effects of solid-liquid ratio and operating pressure on fraetionation of polysaeeharides were investigated.The results showed that S.pallidum polysaeeharides were mainly included in the fractions whose molecular weight was bigger than 50 kD.A higher solid-liquid ratio would lead to less loss of polysaeeharides in membrane separation.It was considered suitable to dilute the solution at the ratio of 1:150 (W/V,g:mL) before membrane separation.The operating pressure had no significant effect on polysaeeharides loss.So the pressure of 0.1~0.5MPa was suitable as long as it was in the allowable scope of membrane separation equipment.%对膜分离技术分级分离马尾藻多糖提取液进行了研究,比较了不同料液比及操作压力对多糖分级分离的影响.结果表明,多糖主要包含在分子量大于50kD的截流液中:随着料液比的减小,膜分离后的多糖损失减少,确定最佳料液比为1:150(W/V,g:mL);操作压力对多糖损失无显著影响,在设备允许压力范围内,0.1~0.5MPa都适合.
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