红麻种质资源SRAP指纹图谱构建及遗传多样性分析

摘要

利用SRAP标记构建51份红麻种质资源的指纹图谱.12对SRAP引物共扩增出167条清晰的谱带,其中165条具有多态性,多态性比率(PPB)为98.8%.51份材料间Nei's基因多态性(Gene diversitv)为0.616 6,平均多态性信息量(PlC)达0.584 2.材料间遗传多样性高.遗传距离较远,亲缘关系较远.SRAP聚类分析结果表明,51份红麻种质资源被聚为5个类群.亲缘关系树状图在分子水平上清晰揭示了红麻种质资源间的亲缘关系,为红麻育种和杂交亲本的选育提供了理论依据,为红麻品种鉴定、遗传改良和分子标记辅助育种奠定了分子生物学基础.%51 accessions of kenaf germplasm from different countries and areas were analyzed using sequence-related amplified polymorphism(SRAP). 12 SRAP primers amplified 167 bands, of which 165 were polymorphic and PPB was 98.8％.Gene diversity and PIC were 0.6166 and 0.5842 respectively. The results showed high genetic diversity,high genetic distance and wide genetic relation. The cluster analysis based on SRAP markers revealed that the kenaf germplasms were clustered into 5 groups. The dendrogram showed clear genetic relationships among the tested kenaf germplasms in molecular level and would provide the theoretical base for breeding and selection of cross-bred parents and lay molecular biological foundation for variety identification, genetic improvement and marker-assisted breeding in kenaf.