The foodborne Escherichia coli O157:H7 could be detected through both Agilent 2100 Bioanalyzer and agarose gel electrophoresis when the specific amplified sequence of the aim gene rfbE was obtained by PCR technology. In this paper,the sensitivity, accuracy, and repeatability of the two methods were compared. The Bioanalyzer method was as 10 times sensitive as the agrose gel electrophoresis method, and it could provide the accurate size. mass and molar concentration of the PCR product. These results showed that the Agilent 2100 Bioanalyzer method could be used for the detection of the foodbome E.coli O157:H7.%根据致病性大肠杆菌O157:H7的致病基因rfbE设计特异性引物扩增出致病性O157:H7特异性序列,然后分别用常规的琼脂糖电泳技术和Agilent 2100 Bioanalyzer对PCR产物进行检测,并比较两种检测方法的灵敏度、准确度和重复性.结果表明,Bioanalyzer的灵敏性较琼脂糖凝胶电泳法高;其能准确地确定片段的大小(误差率小于5%),可提供PCR目的片段的质量浓度和摩尔浓度.Bioanalyzer芯片分析系统结合PCR方法可对食源性大肠杆菌O157:H7进行特异、准确、稳定、灵敏的检测和鉴定,具有重要的推广价值.
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