湖北不同地区甘薯蔓割病病原菌的分离与分子鉴定

摘要

2011~2014年分别从湖北省甘薯主产区十堰市、武汉市、宜昌市等地分别采集了甘薯蔓割病(Fusarium bulbigenum)发病典型植株,在实验室分离纯化并培养,并对分离得到的真菌核糖体基因转录间隔区进行测序分析。测序结果显示目的片段长度为544 bp,武汉和宜昌的序列完全一致。从十堰扩增出来的序列的385位置是C而武汉和宜昌扩增的结果该位置是T,说明引起湖北省甘薯蔓割病的甘薯镰孢菌存在多态性。%From the city of Shiyan, Wuhan, Yichang, 3 Fusarium bulbigenum pathogens were separated and purified. by Molecular identified F. bulbigenum from different regions of Hubei province. The test results showed that the length of the tar-get fragment was 544 bp, and the sequence of Wuhan and Yichang was the same. From Shiyan amplified sequence of 385 position was C and Wuhan and Yichang amplification results of the location was T, molecular indicating that the F. bulbi-genum from different regions of Hubei province presenced polymorphism.