印楝素对黄粉虫幼虫呼吸作用和保护酶活性的影响

摘要

To investigate the effects of botanical pesticide on the respiration and protective enzymes of coleopteran,CO2release rate and protective enzymes in the 8th instar larvae of Tenebrio molitor fed with azadirachtin were examined. The results showed that the CO2release rate and protective enzymes of T. molitor fed with azadirachtin increased initially and then de-clined rapidly. The highest CO2release rate of T. molitor was 13.2 μL/h after 6 hours of feeding with azadirachtin and then fell quickly,remarkably lower than the value of control groups. The maximum activities of protective enzymes including SOD, CAT and POD appeared after 6 hours of feeding with azadirachtin, which were 2.34,2.91 and 1.98 times than those in the control group,respectively. And these protect enzymes lowered fast subsequently.%为了解植物源农药对鞘翅目昆虫呼吸代谢和保护酶活性的影响,以黄粉虫(Tenebrio molitor)8龄幼虫为研究对象,以印楝素为供试农药,采用饲喂法测定印楝素对黄粉虫末龄幼虫CO2释放率和保护酶活性的变化.结果表明,印楝素处理后,黄粉虫的CO2释放率和保护酶活性均先升高后迅速下降.摄入印楝素6 h后,黄粉虫的CO2释放率达到最大值,为13.2 μL/h,之后迅速下降并显著低于对照;保护酶SOD、CAT和POD活性在6 h时达到最大,分别是对照的2.34、2.91和1.98倍,其后呈现下降趋势.