丁香对幽门螺杆菌的体外抑菌作用

摘要

目的 考察丁香挥发油及丁香酚体外抑制幽门螺杆菌的活性.方法 水蒸气蒸馏法提取丁香挥发油,采用高效液相色谱法测定挥发油丁香酚的含量.以氨苄西林为对照,体外法测定丁香挥发油、丁香提取挥发油后的水提取物及丁香酚对8种幽门螺杆菌菌株的最小抑菌浓度(MIC).结果 氨苄西林对8种幽门螺杆菌菌株的MIC范围为1.25～2.50 μg·mL-1,丁香挥发油的MIC范围为5.00～10.00 μg·mL-1,丁香酚的MIC范围为2.50～5.00 μg·mL-1,丁香提取挥发油后的水提取物无抑菌作用.结论 丁香抑制幽门螺杆菌的活性成分是其挥发油中的主要成分丁香酚.%Objective To study the antibacterial effects of eugenol and essential oil from Euggenia Cairyphyllata against helicobacter pylori in vitro. Methods The essential oil was obtained by steam distillation. The concentration of eugenol was determined by HPLC. MIC against helicobacter pylori of eugenol, essential oil from Eugenia Caryophyllata and aqueous extract of Eugenia Caryophyllata except essential oil were determined in vitro with ampieillin as a control drug. Results The range of MIC against 8 helicobacter pylori was 1.25-2.50 μg· mL-1 for ampicillin, 5.00-10. 00 μg· mL-1 for the essential oil, 2.50-5.00 μg· mL-1 for eugenol. The aqueous extract showed no effect against helicobacter pylori. Conclusion The main active component from Eugenia Caryophyllata against H. pylori is eugenol of essential oil.