Objective To study the transdermal characteristics of star anise oil and its percutaneous penetration enhancement for ibuprofen. Methods To establish a method for determination of ibuprofen and anethole , the major constituent of star anise oil by HPLC. Fetal skin was used as permeation barrier in vitro. The transdermal properties of the star anise oil and its effect on percutaneous penetration of ibuprofen were investigated by using Franz diffusion cells . Results The permeability coefficients of star anise oil at 0.5％ ,1.0％ , 2.0％ were (1.675 ± 0.331) , (4.048 ± 0.579) , (5.352 ± 0.166) [μg·(cm) · h , respectively. The permeability coefficients of ibuprofen with star anise oil at 0.0％ , 0.5％ , 1.0％ , 2.0％ were (9.416±1.260) ,(7.803±l.128) ,(9.495±0.749) ,(7.334 ± 0.885) |xg- (cm2)-1- h-1, respectively. Conclusions Star anise oil and ibuprofen could well penetrate through fetal skin in a dose dependent manner . Star anise oil does not show transdermal enhancing effects , while inhibits the percutaneous absorption of ibuprofen in vitro.%目的 考察八角茴香挥发油的经皮渗透性及对布洛芬的促透作用,为制备八角茴香挥发油和布洛芬外用制剂及筛选天然透皮促进剂提供参考.方法 建立布洛芬及八角茴香挥发油中主成分茴香脑的高效液相色谱(HPLC)测定方法.以体外婴儿皮肤为渗透屏障, Franz扩散池法考察八角茴香挥发油经皮渗透性及对布洛芬透皮吸收的影响.结果 0.5%,1.0%,2.0%八角茴香挥发油的透皮速率常数(Jss) 分别为(1.675±0.331),(4.048±0.579),(5.352±0.166) μg·(cm2)-1·h-1.无八角茴香挥发油与0.5%,1.0%,2.0%八角茴香挥发油对布洛芬透皮吸收的Jss分别为(9.416±1.260),(7.803±1.128),(9.495±0.749),(7.334±0.885) μg·(cm2)-1·h-1.结论 不同浓度八角油和布洛芬对人皮肤均具有良好的渗透性,且呈浓度依赖性,但八角油对布洛芬未表现出促透作用,甚至抑制布洛芬经皮渗透.
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