广西尖吻蝮蛇毒K组分抗血管生成活性研究

摘要

Objective To study the anti-angiogenesis effect of fraction K from Guangxi Agkitrodon Acutus venom. Methods The inhibitory effects of fraction K against endothelial cell line ECV304 in vitro was measured by MTT, adhesion of ECV304 cells to fibronectin (FN ) cell by fraction K was observed. The anti-angiogenesis effect in vivo was evaluated by chick embryo chorioallantoic membrane ( CAM) assay. Results The proliferation of endothelial cell line ECV304 was inhibited significantly by fraction K in a time and dose dependent manner. Fraction K also inhibited endothelial cell adhesion to FN and tubule formation in CAM. Conclusion Fraction K has an anti-angiogenesis activity.%目的 探讨广西尖吻蝮蛇毒小分子多肽组分(K组分)对血管生成的抑制作用.方法 采用噻唑蓝法检测K组分对培养的人脐静脉内皮细胞株ECV304的生长抑制情况,结晶紫法测定K组分对ECV304细胞的黏附抑制作用,鸡胚尿囊实验观察K组分对血管生成的影响.结果 K组分对人脐静脉内皮细胞株ECV304的增殖有抑制作用并呈明显的时-效及量-效关系,能够抑制ECV304细胞与纤维连接蛋白的黏附和鸡胚尿囊血管生成.结论 广西尖吻蝮蛇毒K组分对血管生成有抑制作用.