乳癖消颗粒对小鼠体外子宫收缩的抑制作用∗

摘要

目的：探讨乳癖消颗粒对小鼠体外子宫平滑肌收缩活动的影响。方法采用小鼠体外子宫收缩实验，将经过雌激素化的小鼠处死，取出子宫。用生物机能系统记录体外子宫平滑肌收缩曲线，并观察终浓度10，20，30，40，50，60 mg•mL－1乳癖消颗粒溶液对雌激素化小鼠体外子宫的影响和对缩宫素(5 U•L－1)引起的体外子宫痉挛收缩幅度、频率、活力的抑制作用，计算抑制率。结果与基础值相比，乳癖消颗粒溶液在10~60 mg•mL－1终浓度时能显著降低小鼠子宫收缩能力(P<0.01)；其在10~50 mg•mL－1终浓度时显著抑制缩宫素诱导的体外子宫痉挛性收缩(P<0.01)。结论乳癖消颗粒能显著松弛小鼠正常子宫并能拮抗缩宫素引起的子宫平滑肌痉挛。%Objective To study effects of Rupixiao granules on contraction of uterus of mice in vitro. Methods Mouse model with isolated uterine contraction was established. The mice pretreated with estradiol benzoate were sacrificed and then the uteri were taken out. The normal contractions were recorded by the biological function system. Effects of 10, 20, 30, 40, 50, 60 mg.mL-1 Rupixiao granules on spontaneous and oxytocin-induced (5 U.L-1) uterine contractions were recorded,inhibition of Rupixiao granules on mean constriction amplitude,constriction frequency and contraction activity of pitocin-pretreated uterus was observed and the inhibition rates were calculated. Results Compared with the baselines, the amplitude, frequency, activity of spontaneous uterine contraction were significantly down-regulated by Rupixiao solution at a range of 10-60 mg.mL-1 ,as well as it could inhibit oxytocin-induced spasmodic contraction of isolated uterus at 10-50 mg . mL-1 ( P<0. 01 ) . Conclusion The Rupixiao granules inhibits spontaneous contraction of isolated mouse uteri and oxytocin-induced spasm of uterine smooth muscle in vitro.