BMSCs在功能化自组装多肽水凝胶中定向分化神经细胞研究

摘要

Objective:To investigate the potential and differentiation of bone marrow stromal cells which in functional self-assembling peptide hydrogel after directed differentiation for neural cells functional charac-teristics.Method:The functionalized self-assembling peptide was produced by mixing the self-assembling peptide RADA16 and peptide RADA16-IKVAV.BMSCs were seeded into RADA 16 self-assembling peptide hydrogel (control group) and functionalized self-assembling peptide hydrogel (experimental group) surface. Pre-inducing agents with bFGF and EGF and directed inducers ( SHH +RA) induced timing , application Nestin, after MAP2, GFAP, ChAT, VAT immunofluorescence staining to detect morphological and function-al differentiation of neural-like cells mark .Result: By inducing BMSCs in functional self-assembling pep-tide hydrogel culture and inducers communist specific combination and timing of the proliferation and differ -entiation of neuronal cells exhibit like change , MAP2-positive cells was significantly increased compared with the control group ( P<0.05) , GFAP-positive cells the percentage was significantly lower than the con-trol group ( P<0.05) , and the feature flag ChAT , VAT expressed only in the experimental group .Conclu-sion:BMSCs in the function of self-assembling peptide hydrogels can be induced into neural cells have cer-tain features.%目的：探讨骨髓基质干细胞（ BMSCs ）在功能化自组装多肽水凝胶中定向分化为神经细胞及分化后细胞的功能特征。方法：制备功能化自组装多肽水凝胶，分别将BMSCs接种于RADA16自组装多肽水凝胶（对照组）与功能化自组装多肽水凝胶（实验组）表面，观察细胞迁移情况。用预诱导剂bFGF和EGF及定向诱导剂（SHH＋RA）时序诱导，应用Nestin、MAP2、GFAP、ChAT和VAT染色，比较分化后神经样细胞的形态和功能标志的差异。结果：BMSCs在功能化自组装多肽水凝胶中共培养及诱导剂的特定组合和时序的诱导下增殖分化呈现神经元细胞样改变，MAP2阳性细胞百分率较对照组显著提高（P＜0．05），GFAP 阳性细胞百分率较对照组显著降低（P＜0．05），且功能标志ChAT和VAT只在实验组表达。结论：BMSCs在功能化自组装多肽水凝胶中能定向诱导分化为具有功能的神经细胞。