外周血及蜕膜组织中CD4加CD25加调节性T细胞比例与原因不明复发性流产的关系研究

摘要

Objective: To explore the relationship between the proportion of CD4+CD25+regulatory T cells in the peripheral blood and decidua tissue and unexplained recurrent abortion. Methods: Randomly se-lected from June 2012 to June 2015 in our hospital outpatient and hospitalization of patients with unexplained recurrent abortion, 62 cases (observation group) and 62 cases of normal pregnant women (control group) as the research objects. Using flow cytometry four-color fluorescence method to measure the CD4+CD25+regula-tory T cells proportion of peripheral blood and decidua tissue. Comparative analysis of two groups of peripheral blood and decidua tissue proportion of CD4+CD25+regulatory T cells in case. Results:The CD4+CD25+regu-latory T cells proportion of peripheral blood and decidua tissue of observation group patients were significantly lower than control group in patients. The difference between the two groups was statistically significant (T = 2. 669, p = 0.015, p < 0.05); The proportion of CD4+CD25+ regulatory T cells in decidual tissue of observa-tion group patients was significantly lower than that in the control group patients. There was obvious difference between the two groups, with a statistical significance (T = 4.986, p = 0.001, p < 0.05). Conclusion: The proportion of CD4+CD25+regulatory T cells in peripheral blood and decidua tissue has a significant rise in ear-ly pregnancy. It should involve in maintaining normal pregnancy. It is possible that local immune tolerance control of maternal-fetal interface formed an important factor; The lower of the proportion of CD4+CD25+regu-latory T cells in peripheral blood and decidua tissue may be associated with the occurrence of URSA.%目的：：探讨外周血及蜕膜组织中 CD4＋CD25＋调节性 T 细胞比例与原因不明复发性流产的关系。方法：随机选取自2012年6月到2015年6月,我院门诊部与住院部的原因不明复发性流产患者62例(观察组)和正常妊娠妇女62例(对照组)作为研究对象。采用流式细胞四色荧光法测量两组研究对象外周血及蜕膜组织中的 CD4＋CD25＋调节性 T 细胞比例。分析比较两组研究对象外周血及蜕膜组织中 CD4＋CD25＋调节性 T 细胞比例情况。结果：观察组患者外周血中 CD4＋CD25＋调节性 T 细胞比例低于对照组患者外周血中 CD4＋CD25＋调节性 T 细胞的比例,且两组差异具有统计学意义( t ＝2.669,P ＝0.015)；观察组患者蜕膜组织中 CD4＋CD25＋调节性 T 细胞比例低于对照组患者蜕膜组织中CD4＋CD25＋调节性 T 细胞比例,两组差异具有统计学意义(t ＝4.986,P ＝0.001)。结论：外周血及蜕膜组织中 CD4＋CD25＋调节性 T 细胞比例在早孕期显著升高,参与了正常妊娠的维持,有可能是调控母胎界面局部免疫耐受形成的一个重要因素；外周血及蜕膜组织中 CD4＋CD25＋调节性 T 细胞比例降低可能与 URSA 的发生有关。