Objective To observe the effect of different concentrations of glucose and insulin on the secretion of VEGF in 4T1 tumor cells by simulating the changes of glucose and insulin levels at different stages of diabetes mellitus in vitro. Methods The 4T1 tumor cells were cultured in vitro. According to the concentrations of glucose and insulin in culture medium the cells were divided into 15 groups. After different culture media were added,the cells were cultured for 48h. In all experiments VECF concentrations in the supernatant were detected by ELISA. Results As compared with that in group A (control group) ,the concentration of VEGF in group B,C,D and E had no significant differences ( P >0.05). In group H and group I,the levels of VEGF were significantly higher than those in group A. However the concentration of VEGF in group F,G,J had no significant difference,as compared with that in group A respectively ( P > 0. 05 ). In group L and group M, the levels of VEGF were significantly higher than those in group A ( P < 0. 05 ). However, as compared with that in group A, the concentration of VEGF in group K, N, 0 had no significant difference ( P > 0. 05). Conclusion The high concentration of insulin can increase secretion of VEGF from 4T1 tumor cells,however,the concentration of glucose has no obvious effect on the secretion of VEGF from 4T1 tumor cells.%目的 通过模拟糖尿病病程不同阶段患者体内葡萄糖和胰岛素浓度的变化,观察不同浓度的葡萄糖和胰岛素对乳腺癌细胞株4T1肿瘤细胞分泌血管内皮生长因子(VEGF)的影响.方法 体外培养4T1肿瘤细胞,根据培养液中加入的葡萄糖和胰岛素浓度的不同,将实验所用细胞分成15组.培养48 h后收集细胞培养上清,ELISA法检测VEGF的浓度.结果 B、C、D、E组VEGF的浓度与A组（对照组）比较差异无统计学意义(P＞0.05).H、I组VEGF的浓度明显高于A组(P＜0.05),而F、G、J组VEGF的浓度与A组比较差异无统计学意义(P＞0.05).L、M组VEGF的浓度明显高于A组(P＜0.05),而K、N、O组VEGF的浓度与A组比较差异无统计学意义(P＞0.05).结论 高浓度的胰岛素能显著增加4T1肿瘤细胞VEGF的分泌,葡萄糖的浓度对4T1肿瘤细胞分泌VEGF无明显影响.
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