The Decrease of Lung Eosinophil Following Zinc Supplementation in Mouse Models of Respiratory Allergic Inflammation

摘要

Background:United airway disease,a comorbid of allergic rhinitis and asthma,leads to the accumulation of toxic eosinophil in the lung parenchyma as a result of allergic reaction.Ovalbumin(OVA)sets off a condition of eosinophilia in the respiratory tract.Zinc supplementations are proposed to decrease the amount of eosinophil in the lung parenchyma due to its cytoprotective,anti-inflammation and antioxidant activity.Objectives:Proving the effects of the zinc supplementation on the number of eosinophil in the lung histopathology preparations of the BALB/c mice that were sensitized with OVA.Method:A post-test only control group design was used,which consisted of eighteen female BALB/c mice divided into three groups:the negative control group(K1),positive control group with OVA sensitization(K2),and group of mice with OVA sensitization and 5 mg/kg body weight zinc supplementation(P).The treatments were given for 30 days and then eosinophils in the lung histopathology preparations of the BALB/c mice were counted.Results:The mean eosinophil count in the negative control group(K1)was 0.48,the positive control group(K2)was 2.12,and the group of mice with OVA sensitization and 5 mg/kg body weight zinc supplementation(P)was 0.76.The number of eosinophil in the lungs in the K2 group was significantly higher than the K1 group(p=0.000)and the P group was significantly lower than K2 group in ANOVA Test followed by Post Hoc Test(p=0.001).Conclusion:The zinc supplementation reduces the number of eosinophil in the lungs tissue of the BALB/c mice that were sensitized with OVA.