HPLC法测定复方硝酸咪康唑搽剂中醋酸氟轻松和硝酸咪康唑的含量

摘要

目的 建立HPLC法测定复方硝酸咪康唑搽剂中醋酸氟轻松和硝酸咪康唑的含量.方法 采用高效液相色谱法.色谱柱为Wondasil C18(250 mm×4.6 mm,5 μm);以甲醇-水梯度洗脱;检测波长240 nm;流速为1.0 mL/min.结果 醋酸氟轻松和硝酸咪康唑的线性范围分别为0.05~0.30 μg(r=1)和2.5~15 μg(r=1).平均回收率分别为99.39% (n=9,RSD=0.71%)和100.41%(n=9,RSD=1.04%).结论 HPLC法对复方硝酸咪康唑搽剂的含量测定方法是一种有益的补充,本试验方法简便易操作,对目标成分检测具有一定的专属性,可用于复方硝酸咪康唑搽剂的质量控制.%Objective To establish an high-performance liquid chromatography(HPLC)method for determina-tion of fluocinonide and miconazole nitrate in compound miconazole nitrate liniment.Methods HPLC method was ad-opted.The determination was performed on Wondasil-C18 column(250 mm×4.6 mm,5 μm particle size)with metha-nol-water gradient elution at the flow rate of 1.0 mL/min.The detection wavelength was set at 240 nm.Results The lin-earity range was 0.05~0.30 μg(r=1)and 2.5~15 μg(r=1).The average recovery rate was 99.39%(n=9,RSD=0.71%) and 100.41%(n=9,RSD=1.04%).Conclusion Determination of fluocinonide and miconazole nitrate in compound mi-conazole nitrate liniment by high-performance liquid chromatography is simple,fast and reliable.