目的：动态观察燃煤型氟中毒对雌鼠卵巢IL‐1β细胞因子的影响。方法采用自由食用织金病区原煤加拌泥煤烘烤的玉米复制燃煤型氟中毒动物模型（对照组、中氟组、高氟组），动态观察雌鼠染氟期间牙齿变化情况。采用离子选择电极法、高温灰化－氟离子选择电极法测定雌鼠尿氟、骨氟含量。于不同时段（60 d、120 d、180 d ）动情期处死雌鼠，分离卵巢。免疫组化法检测卵巢颗粒细胞内分泌细胞因子IL‐1β的表达。结果（1）成功复制实验动物模型。（2）随着染氟剂量增加及染氟时间的延长，雌鼠氟斑牙症状逐渐加重。（3）雌鼠尿氟、骨氟含量随着染氟剂量的增加及染氟时间的延长而增加。（4）在染氟时间相同的组别（60 d、120 d、180 d ），各染氟组（中氟组、高氟组）与对照组相比卵巢颗粒细胞IL‐1β蛋白表达率均降低（P＜0．05），高氟组低于中氟组（P＜0．05）；染氟剂量相同，随着染氟时间延长，各染氟组（中氟组、高氟组）IL‐1β含量逐渐减少，与对照组相比有统计学意义（ P＜0．05）。（5）与对照组相比，染氟60 d时，中氟组、高氟组雌鼠E2含量随染氟剂量的增加而逐渐增高（ P＜0．05）。染氟120 d、180 d中氟组、高氟组雌鼠E2含量随染氟剂量的增加而逐渐降低，但差异均无统计学意义（ P＞0．05）；随着染氟时间点的变化（60 d、120 d、180 d ），对照组雌鼠E2含量呈从低到高再降低的波动性变化，120 d雌鼠高于60 d和180 d ，且120 d与60 d比较差异有统计学意义（ P＜0．05）。中氟组、高氟组E2含量逐渐减低，但仅有高氟组60 d、120 d分别与180 d比较有统计学意义（ P＜0．05）。结论过量的氟可抑制IL‐1β在卵巢颗粒细胞的表达，致使IL‐1β影响颗粒细胞、P分泌和参与生殖调节的作用也减弱，E2含量减少。这可能是氟中毒致使血清E2降低的机制之一。%Objective To observe dynamicly of coal type fluorosis caused female ovarian cell apop‐tosis and discusses the ovary apoptosis mechanism .Methods SD rat were fed with corn dried by burn‐ing coal coming from endemic fluorosis areas with high fluoride ,to establish the animal model of fluom‐sis .Dynamic observing and recording the changes of female rats'teeth .Determination of fluorine con‐tent of urine and bone with Ion Selective Electrode Method .Executing the rats at different periods (60 d ,120 d ,180 d) ,Separation of ovary :Use immunohistochemical method to observe the expres‐sion of proteins (IL‐1β)in ovary .Results The model of male rats poisoned by NaF has been established successful .The symptoms of dental fluorosis became worse increasing with fluoride content and the exposure time;Female mice urinary fluorine ,fluorine contents in bone with the increase of dye fluo‐rine dose and dye fluoride increased with the extension of time .Dye fluorine groups of ovarian granulo‐sa cells IL‐1βprotein expression were decreased compared with control group .Conclusion Fluorosis re‐straines the expression of IL‐1βin ovarian granulosa cell ,lead to damage granulosa cell and reduce the expression of E2 .
展开▼
