Objective To investigate the effect of HBx siRNA on VEGF gene expression of HepG2 .2 .15 cells .Methods Specific siRNA against HBx was transfected into HepG2 .2 .15 cell .Ex-pressions of HBx and VEGF mRNA before and after transfection were detected using Real-time PCR . Expression of VEGF protein was analyzed by western blot (WB) .Results Real-time PCR testing re-sults showed that the expressions of HBx mRNA were (121 .13 ± 8 .72) ,(83 .17 ± 7 .93) ,(56 .33 ± 6 .17) in experimental group cells after transfection of 24 h ,48h ,72 h .The expressions of VEGF mRNA were(76 .26 ± 7 .16) ,(59 .17 ± 6 .42) ,( 42 .17 ± 4 .33) in experimental group cells after trans-fection of 24 h ,48 h ,72 h .WB testing results showed that the expressions of VEGF protein were (0 .357 ± 0 .025) ,(0 .218 ± 0 .051) in experimental group cells after transfection of 24 h ,48 h .Results of WB and Real-time PCR demonstrated that compared with control group cells ,expression levels of VEGF mRNA and protein were obviously down-regulated after transfection with pSIHBV/X ,and there were statistical significantly differences (P<0 .05) .Conclusion The research demonstrates that inhibition the expression of HBx gene by RNA interference could down-regulate VEGF gene at mRNA and protein level in HepG2 .2 .15 cells .%目的：研究 HBx干扰质粒pSIHBV/X对肝癌细胞HepG2．2．15中VEGF表达的影响。方法将针对HBx基因的干扰质粒载体pSIHBV/X转染HepG2．2．15细胞，用Real-time PCR检测转染前后细胞 HBx、VEGF基因 mRNA 表达，Western blot法检测细胞 VEGF蛋白表达。结果Real-time PCR检测结果显示，转染后24 h、48 h、72 h实验组 HepG2．2．15细胞中 HBx基因mRNA相对表达水平分别为（121．13±8．72）、（83．17±7．930）、（56．33±6．17）；24 h、48 h、72 h实验组HepG2．2．15细胞中 VEGF基因 mRNA 相对表达水平分别为（76．26±7．16）、（59．17±6．420）、（42．17±4．33）；Western blot检测结果显示，24 h、48 h实验组 HepG2．2．15细胞中VEGF蛋白相对表达水平分别为（0．357±0．025）、（0．218±0．051）。Western blot及RT-PCR结果显示，与对照组相比实验组中VEGF mRNA 及蛋白的表达量明显下调，差异有统计学意义（P＜0．05）。结论靶向HBx的siRNA干扰质粒能抑制 HepG2．2．15细胞中VEGF mRNA和蛋白的表达。
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