利用微卫星标记分析4种菊头蝠的遗传多样性

摘要

为揭示贵州菊头蝠、中华菊头蝠、中菊头蝠和小菊头蝠的遗传结构、遗传背景,利用6个微卫星位点,对中国4个菊头蝠群体进行遗传检测,计算各群体的等位基因频率、平均杂合度(He)、平均多态信息含量(PIC)和平均有效等位基因数(Ne),同时分析了各品种群体内和群体间的遗传变异,并根据遗传距离进行了NJ聚类.结果表明,各群体的He、PIC、Ne分别为0.6459～0.7099、0.6193～0.6963、3.2068～3.9143;贵州菊头蝠和中华菊头蝠聚为一类,其次是小菊头蝠,最后是中菊头蝠.%The genetic testing of four Rhinolophus species populations were conducted by using six microsatrllite loci,genetic variation within population of the same species and among different species were analyzed to reveal the genetic structure and background of R. rex, R. sinicus, R. a f finis and R. pusillus.The results showed that He, PIC and Ne of different populations were 0. 6459～0. 7099, 0. 6193～0. 6963 and 3. 2068～3. 9143 respectively. The clustering analysis indicates that four Rhinolophus species can be divided into three groups, R. rex and R. sinicus can be clustered as one group.