柱前衍生HPLC法测定煎炸油及香肠中的生物胺

摘要

为探究煎炸油和香肠中生物胺的提取工艺及定量检测方法,以煎炸油及发酵香肠为样品,采用丹磺酰氯为衍生试剂,建立柱前衍生高效液相色谱法对样品中的生物胺进行测定.结果表明:在设定的试验条件下,煎炸油中并没有检测出生物胺,在取得的2种香肠样品中分别检测出6种和7种生物胺,含量最高为精胺,可达0.118μg/kg.证明,该方法不适用于煎炸油中生物胺的测定,但对香肠产品的测定具有一定可行性.%To explore the extraction process and quantitative determination method of biogenic amines in frying oil and sausages,taking the frying oil and sausages as the samples,using the dansyl chloride as the derivative agent,the biogenic amines in the samples were detected by reversed-phase high performance liquid chromatography (HPLC) with per-column derivatization.The results showed that biogenic amines were not detected in frying oil under the pre-set experimental conditions.But in the two kinds of sausages,six and seven kinds of biogenic amines were detected.Spermine had the highest content which could reach 0.118 mg/g.So,this test method was not suitable for the determination of biogenic amines in frying oils,but feasible in certain degree for the determination of sausage products.