采用气相色谱-质谱联用技术对小叶红叶藤挥发油的化学成分进行分析,并用DPPH·法、ABTS,+法和铁氰化钾还原法对其抗氧化能力进行研究.结果表明:从小叶红叶藤挥发油分离出221个色谱峰,共鉴定64个化合物,占挥发油总量的95.72％,主要成分为dl-薄荷酮(53.43％)、(E)-薄荷酮(14.20％)、5-甲基-2-异丙基-3-环己烯-1-酮(9.87％)、β-萜品烯(8.16％)、β-蒎烯(1.54％)、a-蒎烯(1.18％)等.抗氧化实验中,不同浓度的挥发油对DPPH和ABTS+自由基有较好的清除能力,最高清除率分别达到62.25％和68.11％,但其还原能力较弱,抗氧化能力均具有明显的量-效关系.%The constituents of the essential oils of Rourea microphylla from Guangxi were separated and identified by GC-MS. The scavenging activities on DPPH · radical, ABTS · + radical and reducing power were detected by UV-Vis spectrophotometry. 221 compounds -were isolated and 64 compounds were identified that composed about 95.72% of the total essential oils. The principal chemical constituents of the essential oils were dl-Menthone (53. 43%) , (E)-Menthone(14. 20%) , 2-isopropyl-5-methyl-3-Cyclohexen-l-one(9. 87%) ,β-Terpinene (8. 16%) ,β-Pinene(1. 54%),α-Pinene(l. 18%,et al. These oils possessed good scavenging activities on both DPPH · and ABTS · + radical,the scavenging rate reaching to 62. 25% and 68. 11%,respectively, but showed low activities on reducing power. All antioxidant activity showed a concentration-effect relationship.
展开▼
