毛药忍冬(Lonicera serreana )为忍冬属(Lonicera )植物，其花和果实入药，具有清热解毒、凉散风热之功效，但至今缺乏系统化学成分及药理活性研究.为了寻找毛药忍冬中天然抗氧化活性成分，进一步开发利用忍冬属药用植物资源，该研究以 DPPH 自由基清除法为活性指导，首次对毛药忍冬干燥花蕾75％乙醇提取物的不同极性萃取部位进行抗氧化活性测试，结果发现乙酸乙酯萃取物表现出最强的抗氧化活性(平均清除率为89．45％).进一步应用现代色谱手段(硅胶柱色谱、Sephadex LH-20凝胶柱色谱等)，从毛药忍冬花蕾的乙酸乙酯萃取物中分离单体化合物，运用现代光谱分析技术(MS、1 H-NMR、13 C-NMR、COSY、HSQC、HMBC、ROESY)，并结合文献数据鉴定化合物的化学结构.结果表明：从毛药忍冬干燥花蕾75％乙醇提取物中共分离得到9个化合物，分别鉴定为4个酚酸类化合物：绿原酸(1)、绿原酸甲酯(2)、绿原酸乙酯(3)、咖啡酸(4)；4个黄酮类化合物：木犀草素(5)、木犀草素-7-O-β-D-葡萄糖苷(6)、槲皮素(7)、槲皮素-3-O-β-D-葡萄糖苷(8)；1个甾醇类化合物：β-谷甾醇(9).所有化合物均为从毛药忍冬花蕾中首次分离得到.研究结果可为抗氧化类相关产品的开发提供科学依据.%Lonicera serreana is the endemic species in China from the genus Lonicera .The flowers and fruits of L .serreana were commonly used in the traditional Chinese medicine for the treatment of various diseases,but there was no study on the chemical constituents of L .serreana .To study the chemical constituents with antioxidative activ-ity from the flower buds of L .serreana ,DPPH radical scavenging assay was used for screening the active fractions of 75% ethanol extract.The highest antioxidant activity (89.45%)was observed in the ethyl acetate layer of the extract.Further chemical investigation led to the isolation of nine compounds by modern chromatographic method (chromatography on silica gel and Sephadex LH-20 columns).Their structures were elucidated by modern spectrum analysis (MS,1 H-NMR,1 3 C-NMR,COSY,HSQC,HMBC,ROESY).As a result,four chlorogenic acids,four flavonoids and one sterol compound were identified from the ethyl acetate layer of the extract.All of these compounds were isolated from the flower buds of L .serreana for the first time.The chemical constituent of the flower buds of L .serreana was deeply studied under the active guidance of DPPH radical scavenging assay,therefore this studywould accumulate the fundamental data for antioxidant related products development.
展开▼
