Sweet cherry exhibits the monofactorial gametophytic self-incocompatibility system,the correct evaluation and rational utilization on sweet cherry cultivars have been limited because of the self-incompatibility,therefore,determina-tion of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop produc-tion.With 24 sweet cherry cultivars as materials,using 5 pairs of prunus primer combinations to amplify S-allele-specific PCR for 24 kinds of sweet cherries and S-genotypes of sweet cherry cultivars were identified,the S-gene amplified frag-ments were cloned and searched in GenBank,and combined with 5 primer combinations,S-genotype of 24 cultivars were identified.The conclusion was as follows:the same S genes displayed the same size fragments in electrophoresis;The si-zes of S-gene amplification fragments were S1 800 bp,S3 762 bp,S4 962 bp,S5 300 bp,S6 456 bp,S9 650 bp.And the S genotypes of the tested self-incompatible cultivars were identified that Hongshouqiu and Early Ruby were S1S3;Lapins was S1S4;Ruby was S1S6;Brooks was S1S9;Napoleon was S3S4;Qinlin,Tartarian,Van,Zaodaguo,Lizhu,Meizao, 5-106 and Satonishiki the same as Santina were S3S6;Heizhenzhu,Hongdeng,Samituo and Qinying were S3S9;Victor was S5S9;Mingzhu,Hongmi and Rainier the same as Bing were S6S9.%甜樱桃品种绝大部分自交不亲和,限制了甜樱桃的正确评价和合理利用,因此自交不亲和基因型的鉴定对于生产具有重要意义.以24个甜樱桃主栽品种为材料,用5对蔷薇科李属引物组合对24个甜樱桃品种进行了 S 等位基因的 PCR 扩增,克隆 S 基因的扩增片段,用核酸序列在 GenBank 上搜索,确定了5种 S 基因的核酸序列和大小.结果表明：PruC2＋PruC4R 引物组合扩增效果最好；在琼脂糖凝胶上位置相同的扩增带其核酸序列相同,是同一种 S 基因；5种 S 基因扩增片段的大小分别是 S1为800 bp,S3为762 bp,S4为962 bp,S5为300 bp,S6为456 bp,S9为650 bp；24个甜樱桃 S 基因型是红手球、早红宝石为 S1S3,拉宾斯 S1S4′,红宝石 S1S6,布鲁克斯 S1S9,那翁 S3S4,秦林、泰安大紫、先锋、早大果、丽珠、美早、5-106、左滕锦、桑提娜为S3S6,黑珍珠、红灯、萨米脱、秦樱为 S3S9,胜利为 S5S9,明珠、红蜜、雷尼、滨库为 S6S9.
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