超声-微波协同萃取法提取昆仑雪菊中总黄酮的研究

摘要

The extraction and content determination of flavonoids of Kunlun Chrysanthemum were studied.Ultrasonic-microwave synergistic extraction technique applied in flavonoids of Kunlun Chrysanthemum were obtained.With rutin as the standard substance total flavonoids in Kunlun Chrysanthemum determined by ultraviolet spectrophotometry.The linear relationship of Kunlun Chrysanthemum concentration was A=0.01109C＋0.01012（r=0.9997）,recover was 108.69%,and RSD was 2.82%（n=6）.The contents of total flavonoids in Kunlun Chrysanthemum was 14.25%.The assay method was proved to be simple,convenient,accurate with good reproducibility and can be used for the determination of Kunlun Chrysanthemum.%采用超声-微波协同萃取法,从新疆昆仑雪菊中提取黄酮并以芦丁为标准品,用分光光度法测定了其中总黄酮的含量。芦丁浓度与吸光度的线性关系方程为：A=0.01109C＋0.01012（r=0.9997）,表明芦丁在21.340～59.752μg/mL范围内线性关系良好,测得雪菊中总黄酮的含量为14.25%。该方法简便、可靠、准确,可作为该提取物的质量控制标准。