采用C18色谱柱(4.6 mm×250mm,5μm),以乙腈-水(25:75)为流动相,流速1.0ml·min-1,检测波长277nm,建立反相高效液相色谱法(HPLC)测定小儿肺热咳喘口服液中的连翘苷含量.结果表明,连翘苷在0.4132～4.132μg范围内与峰面积呈良好线性关系,r=0.9999,平均加样回收率为98.02％,RSD为1.24％(n=6).本方法分离度好,干扰小,并具有良好的重复性,适用于小儿肺热咳喘口服液中连翘苷的含量测定.%The HPLC determination method was used to detect the phillyrin in lung-heat cough oral solution for children by C18 ( 4. 6 mm X 250mm, 5μm ), and acetonitrile and water ( 25: 75 ) were used as the mobile phase. The flow was set at 1. 0ml·Min-1 , and the detection wavelength was 277nm. The precision and stability were fine in those conditions. The calibration curve was linear over the range of 0. 4132-4. 132μg for phillyrin ( r =0. 9999 ). The average recovery rate ( n =6 ) was 98. 02% for phillyrin. The proposed method can be used in the quantitative analysis of phillyrin in lung-heat cough oral solution for children.
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