一株抗荔枝病原菌的枯草芽孢杆菌的分离鉴定及其发酵条件的初步研究

摘要

A spore-producing bacilli strain ON-6,which was obtained from health litchi fruit tree rhizosphere soil, was identified to be Bacillus subtilis after routine microbial and 16S rDNA molecular biology detection. Strain ON - 6 had high inhibitory rate to mycelial growth of Penorophythora litchi Chen ex ko et al and Colletrichum gloeosp oriodes Penz,and the ratio were around 92. 3% and 70. 3%, respectively. The suitable culture conditions for strain ON-6 producing antifungal substances were glucose as the carbon source,beef extract and peptone as nitrogen source,the temperature between 32°C and 34°C ,the initial pH value ranged from 7. 0 to 7. 5,the optimal load of 250ml flask containing 25ml medium,and the culture time of 7d.%对从健康果树际土壤中分离得到的一株有较强的抑制荔枝病原菌活性的菌株(ON-6)进行鉴定,以荔枝霜疫霉和荔枝炭疽菌作为指示菌,对该菌株的拮抗能力进行测定,并对其发酵条件进行初步研究.结果表明,菌株ON-6鉴定为枯草芽孢杆菌(Bacillus subtilis),其无菌发酵液对荔枝霜疫霉菌、炭疽菌有明显抑制作用,抑制率分别为92.3％和70.3％,其产拮抗物质的最佳培养条件为:碳源为葡萄糖、氮源为牛肉膏和蛋白胨、温度为32～34℃、初始pH值为7.0～7.5、培养时间为7d、装液量为25ml/250ml.