眉斑并脊天牛纤维素酶性质的研究

摘要

为了了解眉斑并脊天牛(Glenea cantor)纤维素酶的性质,选取以木棉树(Bombax ceiba)为食的眉斑并脊天牛为研究对象,按照IUPAC的标准测定方法,测定和定位眉斑并脊天牛肠道中的内切-β-1,4葡聚糖酶、纤维二糖酶以及滤纸酶活力,考察内切-β-1,4葡聚糖酶的最适pH值和最适温度.结果表明:在眉斑并脊天牛肠道中同时存在以上3种纤维素酶,3种纤维素酶都具有酶活力,并且主要集中在天牛肠道的中肠.内切-β-1,4葡聚糖酶具有广泛的pH值和温度适应范围,最适pH值为9.2,最适温度为50℃.%According to the standard method from IUPAC,the activities of endo-β-1, 4-glu-canase, cellobiase and filter paper enzyme horn Glenea cantor were analyzed and their locations were determined. Optimal pH value and optimal temperature of endo-β-l, 4-glucanase were measured. The results showed that there were activities of all above three cellulases in Glenea cantor. They were mainly located in the midgut. The endo-β-1, 4-glucanase had activity in a broad pH value and temperature range. Optimal pH value and optimal temperature of the endo -β-1,4-glucanase were 9. 2 and 50℃ , respectively.